解放军医药杂志Issue(10)：82-85,4.DOI:10.3969/j.issn.2095-140X.2014.10.023
肺腺癌胸腔积液上清液和细胞块EGFR基因突变检测的临床意义
Detections of EGFR Gene Mutations in Pleural Effusion and Pleural Fluid Cell Blocks of Patients with Pulmona-ry Adenocarcinoma
摘要
Abstract
Objective To investigate the detections of epidermal growth factor receptor ( EGFR) gene mutations in pleural effusion and pleural fluid cell blocks of patients with pulmonary adenocarcinoma, using high-resolution melting ( HRM) in order to provide experimental basis for the detection of EGFR gene mutation with the effective hydrothorax component. Methods A total of 43 samples of the pleural effusion and pleural fluid cell blocks from patients with pul-monary adenocarcinoma during September 2010 and May 2013 were collected, and then DNA was extracted respectively. The mutations of EGFR genes in exon 18, 19, 20 and 21 were detected using HRM method. Results There was no sig-nificant difference in EGFR gene mutations between pleural effusion and pleural fluid cell blocks (P>0. 05). The EGFR gene mutations were found on exon 19 and exon 21, and the mutation on exon 21 was completely consistent between the two components. There was a slight difference on exon 19 mutation. Conclusion It is reliable to detect EGFR gene mu-tation in pleural effusion and pleural fluid cell blocks of patients with pulmonary adenocarcinoma. In case of the false neg-ative cases and tumor heterogeneity, both the results of cytology and HE should be taken into account before the EGFR detection to ensure the reliability of the results.关键词
高分辨率熔解曲线/胸腔积液/肺肿瘤/表皮生长因子受体Key words
High-resolution melting/Hydrothorax/Lung neoplasms/Epidermal growth factor receptor分类
医药卫生引用本文复制引用
孟加榕,潘羡心,郭以河,温路生,刘美莲,禹乐,戴太监..肺腺癌胸腔积液上清液和细胞块EGFR基因突变检测的临床意义[J].解放军医药杂志,2014,(10):82-85,4.基金项目
南京军区医药卫生科研基金课题(10MA076) （10MA076）
福建漳州市科技局资助项目(Z2011066) （Z2011066）
