解放军医药杂志Issue(10)：86-89,4.DOI:10.3969/j.issn.2095-140X.2014.10.024
正交试验法优选大苞雪莲总黄酮的提取工艺
Optimized Extraction Technology of Total Flavonoids from Saussurea Involucrate by Orthogonal Design
摘要
Abstract
Objective To optimize the extraction technology of total flavonoids from Saussurea involucrate. Methods The extraction conditions were optimized by considering the effects of solvent concentration, solvent quantity, extraction time and extraction frequency on extraction, and basing the indexes of the dry extraction rate and content of to-tal flavonoids using L9 ( 34 ) orthogonal design. Results Optimum extracting condition was that 10 times of 70% ethanol was added and extracted for twice with 2 h/each time;total flavonoids content was 12. 53%;Rutin content was 2. 46%;dry extraction rate was 18. 20%. Conclusion The optimized technology is stable, convenient and practical, and it may suit the mass production of Saussurea involucrate.关键词
大苞雪莲/黄酮类化合物/正交试验Key words
Saussurea involucrate/Flavonoids/Orthogonal experiment分类
医药卫生引用本文复制引用
吴金华,马慧萍,王宁,马骏,何晓英,李兰茹,贾正平..正交试验法优选大苞雪莲总黄酮的提取工艺[J].解放军医药杂志,2014,(10):86-89,4.基金项目
国家自然科学基金(81202458) （81202458）
全军医药卫生科研基金课题(LZ13GY07) （LZ13GY07）
全军医药卫生科研基金课题(CLZ11JA06) （CLZ11JA06）
