解放军医药杂志Issue(11)：30-33,42,5.DOI:10.3969/j.issn.2095-140X.2014.11.008
人多巴胺D2受体基因内含子区51103 T/C多态性对基因转录活性的影响
Effect of rs2075652 Polymorphism in DRD2 Third Intron Region on Transcription Activity in Human
摘要
Abstract
Objective To investigate the effect of single nucleotide polymorphism ( SNP ) in 51 103 bp (rs2075652) of Dopamine receptor D2 (DRD2) gene of the third intron region on transcription activity and to further study the effect of gene mechanism on onset risk of post-traumatic stress disorder ( PTSD) in human. Methods The third intron region sequences of DRD2 gene T or C at site 51 103 bp were amplified using whole blood genomic DNA as template, the obtained fragments of 399 bp (the transcription start site is marked as +1) were inserted into pmirGlo re-spectively. The recombinant vector or pmirGlo-Basic vector was co-transfected together with pRL-CMV vector containing renal luciferase reporter gene into primarily cultured HEK293 after identifying with restriction enzyme double digestion and sequencing, and the corresponding expression value of firefly luciferase was detected. Results The correct construc-tion of recombinant expression vector pmirGlo-DRD2-T/ pmirGlo-DRD2-C was confirmed by restriction enzyme double di-gestion and sequencing analysis. The luciferase detection showed that expression of firefly luciferase, when the site of 51 103 bp was C, was significantly higher than that when the site of 51 103 was T (P<0. 01). Conclusion Luciferase re-porter vectors pmirGlo-T /pmirGlo-C can be successfully constructed. The transcription activity of DRD2 gene may be en-hanced when the DRD2 gene of the third intron region 51 103 bp site changes from C to T, and these results may be the basis for further study of the function of DRD2 gene intron region.关键词
多巴胺受体D2/内含子区/荧光素酶活性/基因多态性Key words
Dopamine receptor D2/Intron region/Luciferase activity/Gene polymorphism分类
医药卫生引用本文复制引用
段朝霞,张洁元,陈魁君,李晓霞,王建民,李兵仓..人多巴胺D2受体基因内含子区51103 T/C多态性对基因转录活性的影响[J].解放军医药杂志,2014,(11):30-33,42,5.基金项目
国家自然科学基金资助项目(81101422) （81101422）
