解放军医药杂志Issue(1)：73-77,5.DOI:10.3969/j.issn.2095-140X.2015.01.015
肝癌中NDRG2、AKT2 mRNA及其磷酸化蛋白的表达
Expressions of NDRG2, AKT2 mRNA and Their Phosphorylated Proteins in Human Hepatic Carcinoma Cells
摘要
Abstract
Objective To investigate the expressions of N-myc downstream regulated gene 2 (NDRG2), protein kinase B2 ( AKT2 ) mRNA and their phosphorylated proteins in human hepatic carcinoma cells ( HHCC ) of different pathological grades. Methods The expressions of NDRG2, AKT2 mRNA and their phosphorylated proteins in 30 cases of HHCCs ( group A) and 30 cases of adjacent normal tissues ( group B) were detected, and the correlations were also analyzed. Results The NDRG2 mRNA expression in group A was significantly lower than that in group B (P<0. 05), but there was no significant difference in AKT2 expression between the two groups ( P >0. 05 ) , and expressions of NDRG2 mRNA and AKT2 were not correlated with pathological grades, furthermore there was no statistically significant correlation between NDRG2 mRNA and AKT2 mRNA expressions (P>0. 05). The expressions of two kinds of phospho-rylated NDRG2 proteins were low in the samples in which the phosphorylated AKT2 protein serine474 ( P-AKT2-SER474) was highly expressed, and the expressions of two kinds of phosphorylated NDRG2 proteins were high in the samples where the P-AKT2-SER474 was slightly expressed. Conclusion The mRNA expression can not show the corre-lation between NDRG2 and AKT2. The protein levels show that the NDRG2 phosphorylation does not depend on the AKT2 phosphorylation, and competitive mechanisms may exist in the two processes.关键词
NDRG2/原癌基因蛋白质c-akt/癌,肝细胞/实时聚合酶链反应/印迹法,蛋白质Key words
N-myc downstream regulated gene 2/Proto-oncogene proteins c-akt/Carcinoma, hepatocellular/Real-time polymerase chain reaction/Blotting, western分类
医药卫生引用本文复制引用
吴国强,秦海明,王大全..肝癌中NDRG2、AKT2 mRNA及其磷酸化蛋白的表达[J].解放军医药杂志,2015,(1):73-77,5.基金项目
辽宁省自然科学基金(201204747) （201204747）
