解放军医药杂志Issue(3)：45-49,5.DOI:10.3969/j.issn.2095-140X.2015.03.011
心钠素对多发性骨髓瘤细胞增殖及微小RNA-21表达的影响
Effects of Atrial Natriuretic Peptide on the Proliferation of Myeloma Cells and microRNA-21 Expression
摘要
Abstract
Objective To explore the effects of Atrial Natriuretic Peptide ( ANP) on cell proliferation of multiple myeloma (MM) and its anticancer mechanisms. Methods The MM1. S cells were used in this study, and the effects of different concentrations of ANP on cell proliferation and cell cycle were observed. The protein expressions of natriuretic peptide receptor-A (NPR-A) and NPR-C were detected, and the effect of ANP on microRNA-21 (miR-21) expression in MM1. S cells were also analyzed. Results The 1-100μmol/L ANP inhibited the MM cell proliferation in a dose-depend-ent manner;at the 24th h, ANP had the most potent anti-proliferative activity against MM cells, and then anti-proliferative activity became weaker and weaker with time went by. After ANP affected MM1. S cells for 24 h, compared with those in control group, the cell ratios in G1 phase were increased, while the cell ratios in S and G2 phases were decreased, and the expressions of proliferation index (PI) and miRNA were lower than those in control group (P<0. 01, P<0. 05). The NPR-A and NPR-C receptors were found in MM1. S cells. Conclusion ANP in combination of NPR-A/C receptor has anti-proliferative effect in MM cells by blocking cell cycle in G1 phase and inhibiting the miR-21 expression.关键词
多发性骨髓瘤/心钠素/细胞增殖/微小RNA-21Key words
Multiple myeloma/Atrial natriuretic factor/Cell proliferation/microRNA-21分类
医药卫生引用本文复制引用
吴霞,丁江华,袁利亚,陈国安..心钠素对多发性骨髓瘤细胞增殖及微小RNA-21表达的影响[J].解放军医药杂志,2015,(3):45-49,5.基金项目
国家自然科学基金(81460037) （81460037）
江西省自然科学基金(20122BAB205022) （20122BAB205022）
