解放军医药杂志Issue(3)：79-84,6.DOI:10.3969/j.issn.2095-140X.2015.03.020
福建东南沿海某战区医院MRSA感染特点及基因多态性分型研究
A Study on Characteristics and Genetic Polymorphisms of MRSA Infection in a PLA Hospital in Southeast Coastal Region of Fujian Province
摘要
Abstract
Objective To study the characteristics and genetic polymorphisms of methicillin resistant staphylo-coccus aureus ( MRSA) infection in a PLA hospital in southeast coastal region of Fujian Province, and to provide the mo-lecular biological basis for prevention and control strategies of nosocomial MRSA infection. Methods MRSA strains were initially detected using Cefoxitin indicator paper, and MecA genes were detected using polymerase chain reaction ( PCR) technology, MRSA strains detected from clinical samples were identified, and then genotyping of detected MRSA strains were studied using synthetic primer and random amplification of polymorphic DNA technique ( RAPD ) . Results The MecA genes after PCR detection showed that 42 strains of MRSA strains were isolated from clinical specimens and the medical staff. The MRSA genotypes were differentiated with RAPD technology, and homologous analysis categorized 42 clinical MRSA strains into 12 genotypes, in which 7 strains of Type I, 2 of Type II, 4 of Type III, 4 of Type IV, 7 of Type V, 1 of Type VI, 1 of Type VII, 1 of Type VIII, 8 of Type IX, 2 of Type X, 3 of Type XI and 2 of Type XII. Con-clusion MRSA strains have genetic distances of different sizes of similarity in our hospital, therefore nosocomial cross infection is possible.关键词
耐甲氧西林金黄色葡萄球菌/院内感染/PCR/RAPD/聚类分析Key words
Methicillin-resistant staphylococcus aureus/Nosocomial infection/Polymerase chain reaction/Ran-dom amplification of polymorphic DNA technique/Cluster analysis分类
医药卫生引用本文复制引用
魏智艺,程君涛,李小毅,刘丁井..福建东南沿海某战区医院MRSA感染特点及基因多态性分型研究[J].解放军医药杂志,2015,(3):79-84,6.基金项目
南京军区“十一五”医药卫生基金支持项目(10MA080) （10MA080）
