解放军医药杂志Issue(7)：36-39,4.DOI:10.3969/j.issn.2095-140X.2015.07.010
孕酮对 Aβ25-35诱导的大鼠皮质神经元 tau 蛋白过度磷酸化的抑制作用
Inhibition Effect of Progesterone on Aβ25-35-induced Tau Protein Hyperphosphorylation in Cortical Neurons in Rats
陈则纱 1吴红海 2秦亚彬 1庞鑫鑫 1王晓敏 1李文博 3侯艳宁1
作者信息
- 1. 050017 石家庄，河北医科大学研究生院
- 2. 050082 石家庄，解放军白求恩国际和平医院药剂科
- 3. 050030 石家庄，石家庄凯瑞德医药科技发展有限公司
- 折叠
摘要
Abstract
Objective To investigate the effect and the possible mechanisms of progesterone on Aβ25-35-induced tau protein hyperphosphorylation in cortical neurons of primary rats. Methods Cortical neurons of SD rats were primarily cultured for 10 d, and then were divided into the control group, Aβ25-35 injury group and the progesterone protection group. The Aβ25-35 injury group was treated with 20 μmol/ L Aβ25-35 , while the progesterone protection group was treated with 20 μmol/ L Aβ25-35 and 1 μmol/ L progesterone. Western blotting was used to detect levels of tau phosphorylation and phosphorylation-AKt (p-AKt) expression in cortical neurons of rats. Results Phosphorylated levels on Ser404 and Thr231 points of tau protein in Aβ25-35 injury group were increased compared with those in control group, while the phos-phorylated levels of tau protein in progesterone protection group were decreased compared with those in Aβ25-35 injury group (P < 0. 05). The p-AKt expression was decreased in Aβ25-35 injury group compared with that in control group, while p-AKt expression was increased in progesterone protection group compared with that in Aβ25-35 injury group (P <0. 05). Conclusion Progesterone may attenuate Aβ25-35-induced tau protein hyperphosphorylation by increasing the AKt activity in cortical neurons of rats.关键词
孕酮/Aβ25-35/tau 蛋白质类/磷酸化 AKt/阿尔茨海默病/大鼠, Sprague-DawleyKey words
Progesterone/Aβ25-35/Tau proteins/p-AKt/Alzheimer disease/Rats, Sprague-Dawley分类
医药卫生引用本文复制引用
陈则纱,吴红海,秦亚彬,庞鑫鑫,王晓敏,李文博,侯艳宁..孕酮对 Aβ25-35诱导的大鼠皮质神经元 tau 蛋白过度磷酸化的抑制作用[J].解放军医药杂志,2015,(7):36-39,4.
