湖北民族学院学报（自然科学版）Issue(3)：255-261,7.
华山松不同部位 DNA 提取方法比较研究
Comparative Study on the Different Organization of Genomic DNA Extraction in Pinus armandi Franch
摘要
Abstract
It is the critical first step how to obtain high-quality DNA in molecular biology research.In this paper, their genomic DNA from stem,leaves and endosperm of Pinus armandi Franch were extracted using five methods, which were the classical CTAB method, modified CTAB method, simple CTAB meth-od, SDS micro-extraction method and Zigenhagen method.Then, the extracted DNAs were analyzed by ultraviolet spectrophotometer, agarose gel electrophoresis and standard deviation and standard error of a-rithmetic mean.The results showed:①The modified CTAB method was appropriate for leaves genomic DNA extraction, whose yield was moderate, changes of standard deviation and standard error were lower, and DNA quality was better and stable;②In stem genomic DNA extraction, the yield and the purity of DNA from the Zigenhagen method were highest and its changes of standard deviation and standard error were lowest.Its DNA quality was obviously better and stable;③For endosperm, the yield and the purity of DNA from simple CTAB method was highest, and its changes of standard deviation and standard error were lowest, which indicated that the DNA quality from simple CTAB method was obviously better and stable.Therefore,different methods should be used to extract genomic DNA in the different tissue of one and the same plant.To obtain the best result, the method of DNA extraction need to be explored in prac-tice.关键词
华山松/经典CTAB法/改良CTAB法/简易CTAB法/SDS微量法/Zigenhagen法Key words
Pinus armandi Franch/classical CTAB/modified CTAB/simple CTAB/SDS Micro-Extrac-tion/Zigenhagen分类
生物科学引用本文复制引用
谢冬梅,陈国明,何德,李翠新,张新聪..华山松不同部位 DNA 提取方法比较研究[J].湖北民族学院学报（自然科学版）,2014,(3):255-261,7.基金项目
云南省优势特色重点学科生物学一级学科建设项目（50097505）；云南省应用基础研究计划项目（2008ZC092M）；云南省高校特色专业建设点项目（50116001）． （）
