湖北民族学院学报（自然科学版）Issue(3)：276-281,6.
橘皮中黄酮类化合物的提取工艺及抗氧化性研究
Study on Extraction Flavonoids from the Orange Peel and Antioxidant Activities
摘要
Abstract
The flavonoids were extracted from orange peel, which was controlled with assisted microwave radiation.And the optimal operating parameter was obtained.At the same time, the antioxidant activities of flavonoids from orange peel, hydroxyl radicals (· OH) and superoxide anion (O2·- ) were discussed and compared with Vc.The extraction yield of flavonoids from orange peel was determined by the detecting of UV2050 ultraviolet spectrometer and rutin was as the reference substance.Based on the result of single factor experiment,the orthogonal experimental design method was used in order to find the optimal experi-mental parameters.The optimal experiment parameters are as follows:70%( V ∶V) ethanol as the sol-vent,the extraction time of 180 min, the extraction temperature of 80℃,the ratio solid/liquid of 1 ∶10 and pH of 7.Under the optimal condition, the flavonoids could reach as high as 86.73mg/g.The fla-vonoids from orange peel have the scavenging capacity for hydroxyl radical ( · OH) and superoxide anion (O2·- ).When the concentration of flavonoids reached as high as 0.18mg/mL,the scavenging capacity of · OH was 37.72%and the scavenging capacity of O2 ·- was 28.51%when the concentrations of fla-vonoids were 0.14 mg/mL.The free radical scavenging capacity of Vc was better than that of flavonoids.关键词
橘皮/黄酮类化合物/正交试验/提取工艺/自由基/抗氧化Key words
orange peel/flavonoids/orthogonal experimental design/extraction process/free group/an-tioxidation分类
医药卫生引用本文复制引用
金晶,罗爱民,杨艳,张升晖..橘皮中黄酮类化合物的提取工艺及抗氧化性研究[J].湖北民族学院学报（自然科学版）,2014,(3):276-281,6.基金项目
湖北省自然科学基金项目（21361009）；湖北省高校应用化学战略性新兴产业人才培养计划项目；湖北民族学院2013年大学生科研训练计划项目（2013Z105）． （）
