华北农学报Issue(2)：13-17,5.
生肌因子Myogenin和Myf5真核双表达载体的构建及效果检测
Construction and Effect Test of the Eukaryotic Co-expression Vector for Myogenin and Myf5
摘要
Abstract
Muscle development was specially controlled by the myogenic factors such as myogenin and Myf5 . It is necessary to cotransfect these two myogenic factors into non-muscle cells for further investigating the machanism by which they regulate the muscle specific genes. To gain high transfection efficiency,the coding sequences of myo-genin and Myf5 were cloned successively into the pVITRO2 , an expression vector containing two multiple cloning sites ( MCS) for the convenient cloning of two cDNAs. Sequencing results proved that the cloned myogenin and Myf5 are correct,and the transfection assay suggested they could activate their respective target genes respectively.关键词
生肌过程/双表达载体/生肌因子Key words
Myogenesis/Co-expression vector/Myogenic factors分类
生物科学引用本文复制引用
杜朝,孙洪敏,王英泽,孙国杰..生肌因子Myogenin和Myf5真核双表达载体的构建及效果检测[J].华北农学报,2014,(2):13-17,5.基金项目
河北科技大学博士科研启动基金项目(自然科学 QD200962) （自然科学 QD200962）
国家自然基金青年科学基金项目(31100715) （31100715）
国家自然科学基金面上项目(81171455) （81171455）
