华北农学报Issue(2)：27-37,11.
GPV YZ株非结构蛋白1基因的克隆及序列分析
Cloning and Sequence Analysis of Nonstructural Protein 1 Gene from Goose Parvovirus YZ Strains
摘要
Abstract
To conduct cloning and sequencing of non structural protein 1 ( NS1 ) gene from goose parvovirus YZ strain(GPV YZ) and make sequence alignment and analysis of protein structure and function by bioinformatics,and research the characteristics of NS1 gene and the physicochemical properties and pathogenic mechanism of NS1 pro-tein. The sequences of GPVNS1-YZ gene were obtained by PCR, plasmid cloning and sequencing. Bioinformatics was used to analyze the nucleic acid data,deduced amino acid sequence and phylogenetic trees. The result of se-quence analysis showed that the gene of GPVNS1-YZ protein was 1 881 nt in length,which coded 627 amino acids polyprotein. There was high homology in NS1 gene sequences and the phylogenetic relationships of MDPV recently, when compared with selected GPV-GB,MDPV,ChPV and AAV strains in GenBank. It therefore suggested MDPV and GPV from a common ancestor,the host and different evolution for different branch. The homology segment se-quences corresponding to GPVNS1-YZ contained 231 amino acids and the molecular weight,PI,positively or nega-tively charged amino acid, estimated half-life, instability index, aliphatic index and average hydrophobicity were 26. 446 kDa,6. 04,30,28,5. 5 h,40. 23,78. 01,-0. 43 respectively,and indicated that the protein was a hydro-philic protein. There were nine antigenic peptides and 16 B cell epitopes in the protein respectively,which showed high immunogenicity. However,there existed no transmembrane domain,signal peptide and two disulfide bonds in the protein ,and it might not be secreted into the extracellular membrane of host cell for function. The protein struc-ture study indicated that alpha-helix,beta-sheet,random coil were 30. 74%,17. 32%,51. 95% respectively. There was the virus DNA helicase domain of SF3 helicase superfamily in the tertiary structure. This study indicated that GPV NS1 protein might participate in DNA unwinding process during viral DNA replication,when infection occurs.关键词
鹅细小病毒/非结构蛋白基因/非结构蛋白1/序列分析Key words
Goose parvovirus/Nonstructural protein gene/Nonstructural protein 1/Sequence analysis分类
医药卫生引用本文复制引用
吕春伟,朱峰伟,杨丽金,朱新产..GPV YZ株非结构蛋白1基因的克隆及序列分析[J].华北农学报,2014,(2):27-37,11.基金项目
山东省自然基金项目(ZR2011CM008) （ZR2011CM008）
青岛市科技基金资助项目(12-1-4-5-(2)-jch) （12-1-4-5-(2）
