华北农学报Issue(2):80-84,5.
灰葡萄孢菌核发育相关基因的功能分析
Functional Analysis of a Gene Related to Sclerotia Formation in Botrytis cinerea
摘要
Abstract
One sclerotia-defect mutant of Botrytis cinerea BMH174 was obtained by Agrobacterium tumefaciens-mediated method. The flanking sequence was acquired by TAIL-PCR,and then the T-DNA insertion gene confirmed by Blast in the B. cinerea gene database. The mutant gene was identified BC1G_06945. 1 gene by molecular biolo-gy. The sclerotia formation and pathogenicity in B. cinerea which will facilitate to understand molecular mechanism of conidium development,sclerotia formation and pathogenic in B. cinerea.关键词
灰葡萄孢/T-DNA插入突变体/TAIL-PCRKey words
Botrytis cinerea/T-DNA insertional mutant/TAIL-PCR分类
农业科技引用本文复制引用
司贺龙,赵斌,赵福鑫,邢继红,韩建民,董金皋..灰葡萄孢菌核发育相关基因的功能分析[J].华北农学报,2014,(2):80-84,5.基金项目
河北省科技支撑计划项目(NO.12226507) (NO.12226507)
河北农业大学青年科学基金项目(No. QJ201235 ()
QJ201231) ()