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灰葡萄孢菌核发育相关基因的功能分析

司贺龙 赵斌 赵福鑫 邢继红 韩建民 董金皋

华北农学报Issue(2):80-84,5.
华北农学报Issue(2):80-84,5.

灰葡萄孢菌核发育相关基因的功能分析

Functional Analysis of a Gene Related to Sclerotia Formation in Botrytis cinerea

司贺龙 1赵斌 1赵福鑫 1邢继红 1韩建民 1董金皋1

作者信息

  • 1. 河北农业大学 真菌毒素与植物分子病理学实验室,河北 保定 071001
  • 折叠

摘要

Abstract

One sclerotia-defect mutant of Botrytis cinerea BMH174 was obtained by Agrobacterium tumefaciens-mediated method. The flanking sequence was acquired by TAIL-PCR,and then the T-DNA insertion gene confirmed by Blast in the B. cinerea gene database. The mutant gene was identified BC1G_06945. 1 gene by molecular biolo-gy. The sclerotia formation and pathogenicity in B. cinerea which will facilitate to understand molecular mechanism of conidium development,sclerotia formation and pathogenic in B. cinerea.

关键词

灰葡萄孢/T-DNA插入突变体/TAIL-PCR

Key words

Botrytis cinerea/T-DNA insertional mutant/TAIL-PCR

分类

农业科技

引用本文复制引用

司贺龙,赵斌,赵福鑫,邢继红,韩建民,董金皋..灰葡萄孢菌核发育相关基因的功能分析[J].华北农学报,2014,(2):80-84,5.

基金项目

河北省科技支撑计划项目(NO.12226507) (NO.12226507)

河北农业大学青年科学基金项目(No. QJ201235 ()

QJ201231) ()

华北农学报

OA北大核心CSCDCSTPCD

1000-7091

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