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利用BSA法检测水稻条纹叶枯病高效应抗性位点

张云辉 张所兵 林静 汪迎节 方先文

华北农学报Issue(2):85-88,4.
华北农学报Issue(2):85-88,4.

利用BSA法检测水稻条纹叶枯病高效应抗性位点

Detection of High Effect Site for Resistance to Rice Stripe Virus by BSA

张云辉 1张所兵 1林静 1汪迎节 1方先文1

作者信息

  • 1. 江苏省农业科学院 粮食作物研究所,江苏 南京 210014
  • 折叠

摘要

Abstract

The Jiangsu landrace rice variety Handao was previously identified as high resistance to rice stripe disease (RSV). A total of 141 F2:3 lines derived from the cross of Handao and Wuyujing 3,a variety high sensitive to RSV,were investigated by natural infection method in field,and the disease rating index was scored. Analysis of the resistant/sensitive pools by bulked segregant analysis indicated that the SSR markers RM209 and RM21 on rice chromosome 11 obviously linked to RSV resistance. A QTL for RSV was detected on rice chromosome 11 between RM209 and RM21 by the software QTL IciMapping V3 . 2 based on the principle of inclusive composite interval mapping (ICIM) with the maximum LOD score 13. 25,which explained 38. 39% of the trait variance. Additive effects of qSTV11HD was negative,indicating that the resistance allele was inherited from Handao.

关键词

水稻/条纹叶枯病/群体分离分析法/QTL定位

Key words

Rice/Rice stripe virus/Bulked segregant analysis/QTL mapping

分类

农业科技

引用本文复制引用

张云辉,张所兵,林静,汪迎节,方先文..利用BSA法检测水稻条纹叶枯病高效应抗性位点[J].华北农学报,2014,(2):85-88,4.

基金项目

江苏省农业科技自主创新基金项目[CX(12)2024] (12)

华北农学报

OA北大核心CSCDCSTPCD

1000-7091

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