华北农学报Issue(4)：49-55,7.
玉米 APX 基因的克隆及其原核表达研究
Cloning and Prokaryotic Expression Analysis of APX Gene from Maize
摘要
Abstract
Ascorbate peroxidase ( APX) is a key enzyme which eliminates oxygen free radicals and increases plant resistance in adverse circumstances .In order to study the function of ascrobate peroxidose in cold treated maize leves . The complete open reading frame of APX gene was cloned from cold-stressed (4 ℃) maize inbred line E28 using the method of reverse transcription PCR ( RT-PCR) .The primers were designed according to the published APX cDNA se-quences .The sequence of APX from E28 was 753 bp,encoding a protein of 250 amino acid residues with a predicted molecular weight of 27.3 kDa and a theoretical pI of 5.56.APX were constructed into the expression vector pET-28a (+)with full-length ORF,then transferred into E.coli.After inducing by IPTG,the product proteins of APX were de-tected by SDS-PAGE and the proteins were 27.3 kDa as expected .Salt tolerance analysis showed that the recombinant exhibit strong tolerance to salt than the non-recombinant bacteria ,and the critical concentration of NaCl is 0.6 mol/L. Our researches could lay the foundation for the study of plant tolerance in the future .关键词
APX/原核表达/pET28a(+)/IPTG/BL21(DE3)Key words
APX/Prokaryotic expression/pET28a(+)/IPTG/BL21(DE3)分类
生物科学引用本文复制引用
任瑛,赵美爱,郭新梅,裴玉贺,宋希云..玉米 APX 基因的克隆及其原核表达研究[J].华北农学报,2014,(4):49-55,7.基金项目
青岛市公共领域科技支撑计划项目（12-1-3-15-nsh）；国家自然科学基金项目（31371636）；山东省农业生物资源创新利用研究课题项目；山东省现代农业产业技术体系玉米产业创新团队项目 （）
