华北农学报Issue(4)：111-115,5.
卵丘细胞对牛卵母细胞体外成熟的影响
Effect of Cumulus Cells on in vitro Maturation of Bovine Oocytes
摘要
Abstract
To improve the in vitro maturation rate of bovine oocytes ,the present study was carried out to deter-mine the effects of cumulus cell on in vitro maturation rate of cumulus-oocytes-complexs(COCS)and artificial denu-ded oocytes ( DOs) ,and its pathway and strength was also investigated .The results showed that co-culture with dis-crete cumulus cells significantly improved the in vitro maturation rate of denudes oocytes (57.98 ±14.27 vs 35.53 ± 14.00,P<0.05),but had no effects on COCS (76.66 ±5.77 vs 66.76 ±9.46,P>0.05);Co-culture with sur-rounding cumulus cell did not significantly improve the in vitro maturation rate of denudes oocytes ( 35 .83 ±18 .32 vs 35.53 ±14.00,P>0.05).The analysis results showed that cumulus cell promoted the in vitro maturation of oo-cytes by the pathway of gap-junction ,however discrete cumulus cells worked by the pathway of paracrine;Three or more layers cumulus improved the mean maturation ability of their surrounded oocytes by 85.34%,added discrete cumulus cells separately improved the mean maturation ability of COCs and DOs by 16 .18%and 60 .61%.关键词
卵丘细胞/卵母细胞/体外成熟/牛Key words
Cumulus cells/Oocytes/In vitro maturation/Bovine分类
农业科技引用本文复制引用
黄承俊,郭林林,孙守强,林峰,刘海军..卵丘细胞对牛卵母细胞体外成熟的影响[J].华北农学报,2014,(4):111-115,5.基金项目
天津市科技支撑计划重点项目 （）
