华北农学报Issue(4)：135-139,5.
VNTR 分子标记技术在甜菜不育系选育中的应用
VNTR Molecular Marker Technology in the Application of Beet Sterile Lines Breeding
摘要
Abstract
In order to utilize heterosis more efficiently ,select and maintain pairwise pure beet male sterile lines and maintainer lines .In this study ,we used the unstable beet male sterile lines and their corresponding maintainer lines in bagging selection process as experimental materials .Special primers have been developed for the cytoplasm fertility molecular identification .The results showed that:the special primers can identify male sterile lines and maintainer materials effectively .The primers used in this study can provide more effective service to the sugar beet breeding .We also optimized the extractive method of mitochondrial DNA in sugar beet .And we directly extract the genome DNA for molecular identification .It can simplify the experimental process and improve the efficiency .We i-dentified 160 pairs of germplasms of unstable beet cytoplasmic male sterility through VNTR molecular markers .The 40 pairs of stable pure beet sterile lines and their corresponding maintainer lines were identified .The 40 pairs of stable pure beet are 31303(04)(1 pair),31327(28)(1 pair),31329(30)(1 pair),31333(34)(1 pair),31339(40) (1 pair),31341(42)(5 pairs),313343(44)(5 pairs),31345(46)(4 pairs),31355(56)(2 pairs),31361(62) (1 pair),31365(66)(1 pair),31367(68)(1 pair),31373(74)(1 pair),32315(16)(1 pair),32321(22)(4 pairs), 32323(24)(1 pair),32327(28)(4 pairs),32331(32)(3 pairs),32341(42)(1 pair),32343(44)(1 pair).关键词
甜菜/分子辅助育种/雄性不育株/分子标记Key words
Sugar beet/Molecular assisted breeding/Male sterile line/Molecular marker引用本文复制引用
王良,白晨,李晓东,张惠忠..VNTR 分子标记技术在甜菜不育系选育中的应用[J].华北农学报,2014,(4):135-139,5.基金项目
现代农业产业技术体系建设专项资金项目 （）
