华北农学报Issue(5)：5-9,5.DOI:10.7668/hbnxb.2014.05.002
普通野生稻矮化突变体的株高与分蘖基因的QTL 定位及主效基因的遗传分析
Mapping Quantitative Trait Loci Associated with Height and Tillers of Oryza rufipogon Griff.Dwarf Mutant and Genetic Analysis of Major Quantitative Locus
摘要
Abstract
In order to exploit and isolate more genes controlling plant height and effective tillers , and applies these genes into breeding practice .In this paper ,an F2 isolated population was constructed derived from a cross be-tween a multi-tillering dwarf wild rice mutant and minor-tillering tall Nantehao variety;plant height and effective tillers of the F2 population were investigated and their genotypes were tested ,and QTLs linkage genetic analysis was conducted by using IciMapping V 3.0 soft.Plant height and effective tillers of the two parents were all quantitative traits controlled by multi-genes and had obvious positive relation .194 pairs of molecular markers were identified to have polymorphism between the parents by screening 345 pairs of molecular markers even distributing on 12 chro-mosomes in rice and the polymorphic ratio was of 56.23%.In the F2 population,33 height QTLs and 19 tillers QTLs were obtained using linkage analysis of 122 pairs of molecular markers .One major QTL controlling height was identi-fied and located in the interval markers of RM 302 and RM104 on chromosome 1.The major QTL explained 71.72%phenotypic variation .In our studies , a major QTL controlling plant height was obtained which was closely linkage with molecular markers of RM302 and RM104.It had very strong dwarfed ability .Our results would provide a theo-retical basis for cloning dwarf genes and molecular breeding of constructing ideal plant shape .关键词
水稻/野生稻/株高/分蘖/QTLs/遗传分析Key words
Rice/Oryza rufipogon/Height/Tillers/QTLs/Genetic analysis分类
生物科学引用本文复制引用
王兰,李智,郑杏梅,蔡英钦,罗敏,聂益勇..普通野生稻矮化突变体的株高与分蘖基因的QTL 定位及主效基因的遗传分析[J].华北农学报,2014,(5):5-9,5.基金项目
国家自然科学基金青年项目（30800597）；教育部博士点基金项目（20094404120018）；广东省大学生创新项目（1056412068）；国家公益性行业（农业）科研专项 （）
