华北农学报Issue(5)：10-15,6.DOI:10.7668/hbnxb.2014.05.003
玉米大斑病菌交配型基因克隆及生物信息学分析
Cloning and Bioinformatics Analysis of MAT G enes in Setosphaeria turcica
摘要
Abstract
In this study,we used the strains F 1-40(Mating type A) and 01-23(Mating type a) as materials.In order to obtain the full-length and the flanking sequence of MAT genes,the MAT1 gene of mating type A strain and the MAT2 gene of mating type a strain of Setosphaeria turcica were cloned by PCR and genome walking with the can-didate gene strategy .The conserved domain database of the full-length genes were analyzed .The flanking sequence of MAT1 and MAT2 genes were comprised by the Blast of genomic database of S.tucrica.The results suggested that both genes contain one introns .MAT1 encodes amino acids contain a complete MAT αd-omain,the domain as part of the MAT alpha1 family.MAT2 gene encodes amino acids ,including a complete MATA HMG-box combining with do-main,the domain as part of the DNA binding protein in the HMG -boxⅠclass members of the family ,such member containing a single HMG box ,composed of three helix ,which located between the 133-202 residues in the form of highly sequence specificity combined with DNA minor groove participating in the process of copy transcription and translation of DNA.Then participate in the process of sexual reproduction .Comparing the flanking sequence of MAT1 and MAT2 genes,we discovered the similarity was higher than 93%.关键词
玉米大斑病菌/交配型/基因克隆/生物信息学分析Key words
Setosphaeria turcica/Mating-type/Gene clone/Bioinformatics analysis分类
生物科学引用本文复制引用
刘微微,郭丽婕,贾慧,曹志艳,高瑞萍,韩建民,董金皋..玉米大斑病菌交配型基因克隆及生物信息学分析[J].华北农学报,2014,(5):10-15,6.基金项目
现代农业产业技术体系专项资金项目 （）
