华北农学报Issue(5)：23-28,6.DOI:10.7668/hbnxb.2014.05.005
甘蓝基因组细菌人工染色体文库的构建
Construction of a Bacterial Artificial Chromosome Library from Brassica oleracea
摘要
Abstract
Bacterial artificial chromosome library is the foundation of important and high quality disease -resist-ant gene cloning from Brassica oleracea.A bacterial artificial chromosome library was constructed from R 4P1, a Brassica oleracea varieties highly resisting to fusarium wilt and being in rich of glucosinolates ,with the vector Copy-ControlTM pCC1BACTM.The library consists of 73 344 clones with an average insert size of about 97 kb,and in which the empty clones are under 3%.Thus,the library provides 10.9 haploid genome equivalents ,the probability of screen-ing to any gene of B.oleracea is 99.99%,these results indicate that this is a high quality BAC library suitable for the following analysis .The BAC library of B.oleracea could not only be used for the clone of resistant genes to fusarium wilt,but also lay foundations for isolating other interesting genes and research the genomics of B.oleracea,et al.关键词
甘蓝/细菌人工染色体文库/枯萎病基因/克隆Key words
Brassica oleracea/Bacterial artificial chromosome library/Fusarium wilt resistant gene/Clone分类
生物科学引用本文复制引用
邢苗苗,田多成,李海龙,姚丽君,冀瑞琴,康俊根..甘蓝基因组细菌人工染色体文库的构建[J].华北农学报,2014,(5):23-28,6.基金项目
国家自然科学基金项目（31171958）；“十二五”国家科技支撑计划项目（2012BAD02B01；2012BAD50G01）；国家高技术研究发展计划项目“863”项目 （）
