华北农学报Issue(5)：29-32,4.DOI:10.7668/hbnxb.2014.05.006
利用 cDNA-SRAP 分离结球甘蓝抗黑腐病相关基因的研究
Studies on Isolation of Resistance Related-genes to Black Rot by cDNA-SRAP in Cabbage
摘要
Abstract
In order to explore the resistant disease gene and find out relative mechanisms ,the suspensions with 1.0 ×108 colony forming units/mL was sprayed to the resistant variety C 7 of seedlings at four to five leaves ,while the other group was sprayed distilled water as usual (Control,CK).Leaves were collected in 1,3,5 d time points af-ter XCC and distilled water .A mixture of an equal amount total RNA from the above two groups as two gene pools . Different expression of the genes was analyzed by SRAP technique .The results showed:using 60 pairs of primers ,a-bout 685 cDNA fragments in size between 100~750 bp were amplified ,averagely 11 bands per primer combination , there were 24 polymorphic loci in these two pool .They were sequenced and the Blast program was used to find out homology with reported genes .Sequence alignment indicated that 6 out of these 9 TDFS ( Transcript derived frag-ments) showed homologies to certain genes from 43%to 59%in NCBI,while the other three showed no significant homology with reported gene .Based on their homologies ,these genes were assumed to NADH dehydrogenase ,vegeta-tive cell wall protein ,DELTA-VPE and unknown functional proteins .The results indicated that cDNA-SRAP method had several advantages over other systems:simplicity , reasonable throughput rate , highly reproducibility and inex-pensiveness ,and that the cDNA-SRAP technique was applicable to the analysis of gene different expression .Some fragments of differentially expressed genes associated with cabbage black rot were obtained ,and may be used for in-vestigating the molecular mechanism of cabbage black rot .关键词
甘蓝/黑腐病/cDNA-SRAPKey words
Cabbage (Brassica oleracea L.var.capitata)/Black rot (Xanthomonas Campestris pv.Campes-tris)/cDNA-SRAP分类
农业科技引用本文复制引用
张云霞,宋立晓,曾爱松,高兵,严继勇..利用 cDNA-SRAP 分离结球甘蓝抗黑腐病相关基因的研究[J].华北农学报,2014,(5):29-32,4.基金项目
“十二五”农村领域国家科技计划项目（2012BAD02B00）；江苏省农业科技支撑计划重点项目（ BE2013429）；江苏省农业科技自主创新资金项目（CX（13）2004） （）
