华北农学报Issue(5)：66-70,5.DOI:10.7668/hbnxb.2014.05.012
Y 系山定子 AP1同源基因的克隆及序列分析
Cloning and Analysis of the AP1 Homology Gene from Y Series Malus baccata (L.) Borkh
王娟 1田建保 2杜俊杰 1杨廷桢 3王骞 1孙俊杰 1弓桂花1
作者信息
- 1. 山西省农业科学院 果树研究所，山西 太谷 030800
- 2. 山西农业大学 园艺学院，山西 太谷 030801
- 3. 山西农业大学 园艺学院，山西 太谷 030801
- 折叠
摘要
Abstract
The study of AP1 gene has theoretical value on shortening juvenility of woody fruit plants .According to the reported AP1 gene sequence of apple primers were designed to amplify the AP1 gene of M.baccata using PCR method.The fragment obtained was 1 010 bp in length.The purified PCR products were connected to the pMD 18-T vector.Then the products were transformed into E.coli strain DH5αcompetent cells to obtain recombinant plasmid through the blue-white screening plasmid length and restriction enzyme digestion .Sequencing and sequence analysis showed that:we obtained a middle fragment of AP1 gene,which has two exons ,one introns and the part of two in-trons.The code area altogether codes 81 amino acids,and the 13-81 amino acids is K-region of the MADS-box gene family-specific .After the amino acid sequence carried out on the homology search in GenBank , the results showed that the amino acid sequence homology with other plants is 67 .9%-100%.These results laid the founda-tion for the molecular mechanism of early-flowering phenomenon .They might have both theoretical value and practi-cal significance in fruit tree breeding .关键词
Y系山定子/AP1( APETALA1)基因/PCR/克隆/序列分析Key words
Y series Malus baccata/AP1(APETALA1) gene/PCR/Cloning/Sequence analysis分类
生物科学引用本文复制引用
王娟,田建保,杜俊杰,杨廷桢,王骞,孙俊杰,弓桂花..Y 系山定子 AP1同源基因的克隆及序列分析[J].华北农学报,2014,(5):66-70,5.
