华北农学报Issue(5)：85-91,7.DOI:10.7668/hbnxb.2014.05.015
鸡小肠上皮干细胞的分离培养
Study on Isolation and Culture of Intestinal Epithelial Cell in Chicken Embryo
摘要
Abstract
This current study was conducted to investigate the biological characteristics of chicken IECs ( Intes-tinal epithelial stem cells)by isolating and culturing in vitro.IECs were isolated from small intestine of 18 day-old chicken embryos .The suitable culture medium for IECs was DMEM/F12 .Then its proliferation was analyzed by subculture and the growth curve .The purified chicken intestinal epithelial stem cells were identified by morphology , immunohistochemical methods and RT-PCR.The results showed that chicken IECs can be cultured in vitro and have been passed to 11 generation .The purified IECs were positive by morphology , immunohistochemical methods and RT-PCR.To sum up,IECs can be isolated from small intestine ,which can proliferate rapidly in vitro.关键词
鸡/小肠上皮干细胞/分离培养/鉴定Key words
Chicken/IECs/Isolation and culture in vitro/Identification分类
农业科技引用本文复制引用
裴沛,熊慧,崔鹏,关伟军,马月辉..鸡小肠上皮干细胞的分离培养[J].华北农学报,2014,(5):85-91,7.基金项目
中国农业科学院科技创新工程项目（ASTIP-IAS01）；国家家养动物种质资源平台项目（2014）；转基因生物新品种培育科技重大专项 （）
