华北农学报Issue(5)：107-113,7.DOI:10.7668/hbnxb.2014.05.018
茉莉花粉离体培养萌发及花粉管生长特性研究
Pollen Germination in vitro and Pollen Tube Growth of Jasminum sambac Aiton
摘要
Abstract
To reveal pollen viability in jasmine and provide an efficient method for the detecting ,the effects of sampling stage ,medium type ,concentrations of different additions ,pH value ,culture temperature ,light intensity and time on the pollen germination and pollen tube growth were investigated .Meanwhile ,the method was identified fur-ther through observing the pollen germinated on stigmas under scanning electron microscopy .The results showed that pollen of the highest viability should be collected at 8:00 -9:00 am, the optimal medium was BK ( Containing H3BO3100 mg/L,KNO3 100 mg/L,MgSO4·7H2O 200 mg/L and Ca(NO3)2·4H2O 300 mg/L) added with sucrose 80 g/L and PEG4000 120 g/L,with the suitable pH value of 6.0,temperature of 25-30 ℃,light intensity of 35-50 μmol/( m2· s) and culture time of 3-4 h.Correlation analysis showed that pollen germination and pollen tube growth were very significantly positively correlated with the indices of light intensity and culture time ( P<0 .01 ) , positively with the pH value ,negatively with the concentrations of sugar and PEG 4000 ,and negatively and positively with the temperature ,respectively ,at no significant levels ( P>0 .05 ) .The research shows that jasmine pollen has a low viability on the whole ,and germination in vivo was indeed a simple ,quick and reliable method to determine the viability .But much attention should be paid to the selection and optimization of culture conditions to obtain the best effectiveness .关键词
茉莉/花粉活力/花粉培养/花粉管生长/扫描电子显微镜Key words
Jasmine/Pollen viability/Pollen culture/Pollen tube growth/Scanning electron microscopy分类
农业科技引用本文复制引用
邓衍明,叶晓青,梁丽建,贾新平..茉莉花粉离体培养萌发及花粉管生长特性研究[J].华北农学报,2014,(5):107-113,7.基金项目
国家自然科学基金项目（31101569）；江苏省农业科技自主创新资金项目（CX（11）4032） （）
