华北农学报Issue(6)：1-5,5.DOI:10.7668/hbnxb.2014.06.001
一个新的白菜苗期TuMV-C4抗性主效QTL定位及连锁分子标记开发
Development of Molecular Markers Linked to a Novel QTL for TuMV-C4 Resistance in Brassica rapa L. ssp. pekinensis
摘要
Abstract
Our research constructed a molecular genetic map with a line 91-112 ( highly resistant to TuMV-C4),a highly susceptible line T12-19 and the DH population derived from microspore culture of F1(91-112 × T12-19). A total of 45 markers were mapped to the A09 chromosome,and a new QTL-BrTuA09 controlling TuMV-C4 re-sistance was identified. On this basis,27 pairs of primers were designed by screening the InDel loci on A09 chromo-some of Chinese cabbage based on genome re-sequencing. The results showed that distinct PCR amplification prod-ucts were obtained from the InDel markers. Among these markers,11 InDel were polymorphic on the tested parent materials,and mapped to the A09 chromosome. By linkage analysis,these markers were tightly linked to the QTL-BrtuA09 . The studies showed that these markers can be applied in marker-assisted selection in Chinese cabbage hy-brid breeding programs and improve the resistance to TuMV-C4 .关键词
大白菜/QTL/TuMV/分子标记Key words
Chinese cabbage/QTL/TuMV/Molecular markers分类
生物科学引用本文复制引用
田希辉,于拴仓,苏同兵,张凤兰,余阳俊,张德双,赵岫云,汪维红..一个新的白菜苗期TuMV-C4抗性主效QTL定位及连锁分子标记开发[J].华北农学报,2014,(6):1-5,5.基金项目
科技部科技支撑项目(2012BAD50G01 （）
2012BAD02B01) （）
科技部“863”项目(2012AA020103 （）
2012AA100103) （）
国家大宗蔬菜产业技术体系(CARS-25-A-11) （CARS-25-A-11）
