华北农学报Issue(6)：6-10,5.DOI:10.7668/hbnxb.2014.06.002
水稻中脉缺陷突变体dl-6的遗传分析与精细定位
Genetic Analysis and Gene Fine Mapping of a Midrib-deficient Mutant dl-6 in Rice
摘要
Abstract
To clone the DL-6 locus,positive and negative cross combinations were developed between dl-6 and 9311,and genetic analysis found that drooping leaf was controlled by one recessive nuclear gene DL-6. DL-6 was primarily mapped on the short arm of chromosome 3 with SSR markers. With newly developed markers DL-6 was fi-nally fine mapped in 85 kb region between markers I3-5 and I3-8 . Open reading frame ( ORF ) analysis revealed that ORF9 might be a drooping leaf related gene. ORF9 both in mutant dl-6 and in wild type were sequenced and 1 bp mutation was found in first exon in dl-6 when blasted with Nipponbare. Cysteine mutated into arginine in dl-6. Meanwhile,8 bp deletion was also found at 3′end of YABBY . These two mutations which one is the functional mu-tation of DL-6 is still uncertain and need deeply research.关键词
水稻/中脉缺陷/基因定位/dl-6Key words
Rice/Midrib-deficient/Gene mapping/dl-6分类
生物科学引用本文复制引用
王军,王婧,杨杰,赵祥强,朱金燕,范方军,李文奇,王芳权,仲维功..水稻中脉缺陷突变体dl-6的遗传分析与精细定位[J].华北农学报,2014,(6):6-10,5.基金项目
国家科技支撑计划重大项目(2011BAD16B03) （2011BAD16B03）
江苏省研究生创新工程项目(CXZZ12_0906) （CXZZ12_0906）
江苏省农业自主创新资金项目(CX(12)1003) （CX(12）
江苏省自然科学基金项目(BK20130725) （BK20130725）
江苏省科技支撑计划项目(BE2012309) （BE2012309）
