华北农学报Issue(6)：63-67,5.
猪传染性胃肠炎病毒S基因不同编码区的表达和反应原性鉴定
Prokaryotic Expression and Immunoreactivity of Different Encoding Regions of the Spike Gene of Porcine Transmissible Gastroenteritis Virus
摘要
Abstract
To identify immunoreactivity of different encoding regions of the spike ( S ) gene of Transmissible gastroenteritis virus ( TGEV) ,three encoding regions containing four antigenic sites C,B,D and A located at the N-terminus of S protein was analyzed and expressed by prokaryotic expression system. The fragments TCB ( containing antigenic sites C and B,411 bp) ,TD ( containing antigenic site D,441 bp) and TA ( containing antigenic site A, 456 bp) were amplified by PCR. After cloning and sequence,the fragments were inserted into the prokaryotic ex-pression vector pET-32a respectively. The fusion proteins yielded by TCB,TD and TA recombinant expression plas-mids were 34. 6,35. 1,36. 1 kDa respectively. The expressed proteins showed strong positive reaction with TGEV-infected swine serum and His tag antibody by Western Blot. These results indicated that the three encoding regions of S gene had a strong immunoreactivity with TGEV positive antiserum,which will play an essential role in identif-ying immunogenicity and immunological protection of different encoding regions of the S protein of TGEV.关键词
猪传染性胃肠炎病毒/S基因/原核表达/反应原性Key words
Porcine transmissible gastroenteritis virus/Spike gene/Prokaryotic expression/Immunoreactivity分类
生物科学引用本文复制引用
周宏专,王金洛,覃湘婕,杨兵,徐福洲,满坤,苏霞,张晓东,刘爵,罗绪刚..猪传染性胃肠炎病毒S基因不同编码区的表达和反应原性鉴定[J].华北农学报,2014,(6):63-67,5.基金项目
北京市科技计划项目(No. Z131100003113015) （No. Z131100003113015）
北京市农林科学院博士后基金项目 （）
