华北农学报Issue(z1)：262-272,11.DOI:10.7668/hbnxb.2014.S1.050
丽江新团黑谷近等基因系孕穗期耐冷性生化性状的主成分分析及综合评价
Principal Component Analysis and Comprehensive Evaluation on Biochemical Characteristics of Cold Tolerance at Booting Stage in the Near-isogenic Lines from Japonica Rice Lijiangxintuanheigu
摘要
Abstract
The biochemical evaluation system were established for screening and identifying species with cold tolerance,105 rice materials,including three standard varieties of strong cold resistance from Yunnan Province ,the NILs BC4 F8 and BC4 F9 generation derived from parents japonica cultivars Lijiangxintuanheigu ( donor parent ) and Towada ( recurrent parent ) ,and their parents ,were employed for evaluation of cold tolerance and screening of cold resistance index at booting stage .26 biochemical traits including leaves ,anthers,and grains of tested materials were determined under cold water-stress and normal conditions in 2011 ,2012 .Comprehensive assessment of cold resist-ance based on the principal component analysis , subordinate function values analysis , regression analysis and K-means clustering analysis .The results showed Chlorophyll a ,Chlorophyll b ,total Chlorophyll ,Chlorophyll c content , peroxidase(POD) activity,free amino acid content of flag leaves;soluble protein,soluble sugar,malondiadehyde (MDA) content,catalase(CAT) activity,ascorbic acid(ASA),proline content(Pro) of flag leaves and anthers;sol-uble protein,soluble sugar,protein,γ-aminobutyric acid(GABA) content of grains significantly influenced the cold resistance .Thus could be used as comprehensive index for identification of cold resistance at the booting stage .The values cold resistance comprehensive evaluation ( D) of biochemical traits including leaves ,anthers,and grains of tested materials based on the subordinate function values was chosen as object under cold stress condition ,through K-means clustering analysis ,105 lines of population should be divided into 3 categories including stongly cold-toler-ant lines 32 ,mediate cold tolerance lines 10 ,and cold-sensitive lines 63 .关键词
水稻/近等基因系/孕穗期耐冷性/生化鉴定指标/主成分分析/综合评价Key words
Rice/Near-isogenic lines/Cold tolerance at booting stage/Biochemical identification index/Princi-pal component analysis/Comprehensive evaluation分类
农业科技引用本文复制引用
杨树明,曾亚文,王荔,张素华,杜娟,杨涛,普晓英,杨晓梦..丽江新团黑谷近等基因系孕穗期耐冷性生化性状的主成分分析及综合评价[J].华北农学报,2014,(z1):262-272,11.基金项目
国家自然科学基金项目（30160043；31060186）；云南省自然科学基金项目（2010CD006）；云南省技术创新人才培养项目（2011CI059；2012HB050）；云南省农业生物技术重点实验室开放项目 （）
