华北农学报Issue(1):90-96,7.DOI:10.7668/hbnxb.2015.01.015
分子标记辅助选择聚合Xa23、Stvb-i、Pi-13种抗病基因的研究
Study of Polymerization of Three Disease-resistant Genes by Molecular Marker-assisted Method
摘要
Abstract
In order to breed new disease-resistant varieties and ensure stable and high-yields of rice ,the study on 378 anther culture descendants produced by conventional and anther culture technology was carried out using molecular marker-assisted method.The results showed that three resistance genes of Xa23,Pi-1 and Stvb-i could be polymerized quickly by molecular marker-assisted method .Though the PCR detection and identification of disease resistance ,10 new seeds with stabilized hereditary and high level expression were obtained .The use of molecular marker assisted selection of genotypes at the same time ,still needed to phenotype and artificial identification of re-sistance of the traditional combination ,so as to ensure the high accuracy of selected .关键词
水稻/分子标记辅助选择/花药培养/基因聚合/抗病Key words
Rice/Marker-assisted selection/Anther culture/Gene pyramiding/Resistance分类
农业科技引用本文复制引用
孙海波,邹美智,任洪岩,王景余,闫双勇,李艳萍,冯瑞光..分子标记辅助选择聚合Xa23、Stvb-i、Pi-13种抗病基因的研究[J].华北农学报,2015,(1):90-96,7.基金项目
天津市应用基础研究计划一般项目 ()