华北农学报Issue(1)：118-122,5.DOI:10.7668/hbnxb.2015.01.019
AcMNPV介导BmKIT和组织蛋白酶协同表达对Sf9细胞凋亡的分析
Analysis of AcMNPV Meditated Expression of BmK IT and Vcath on the Apoptosis of Sf9 Cells
摘要
Abstract
In order to investigate the mechanism underlying the BmK IT mediated insecticidal activity of AcM-NPV,an excitatory insect toxin ,BmK IT,gene was inserted into the geneome of AcMNPV to construct a recombinant baculovirus.The scorpion insect neurotoxin BmK IT and vcath gene were constructed into the genome of AcMNPV using Bac-to-Bac system.Effects of BmK IT under these promoters of AcMNPV on Sf 9 cells were analyzed and it re-vealed that the expression level of BmK IT was high under the regulation of PH promoter at 36 ,48 h after the virus infected.Effects of AcMNPV mediated BmK IT and vcath synergism expression on the proliferation of Sf 9 cells was also analyzed.Compared to AcMNPV-BmK IT (P10),the inhibition rate of AcMNPV-BmK IT (P10)-vcath(PH) increased 14.5%averagely,the expression level of c-Myc,cleaved-Caspase3 and Bax was raised and the expression of Bcl-2 was reduced .关键词
AcMNPV/BmK IT/组织蛋白酶/协同作用Key words
AcMNPV/BmK IT/Vcath/Synergy分类
生物科学引用本文复制引用
赵洁,梁爱华,付月君..AcMNPV介导BmKIT和组织蛋白酶协同表达对Sf9细胞凋亡的分析[J].华北农学报,2015,(1):118-122,5.基金项目
国家自然科学基金项目（31272100）；山西省自然科学基金项目 （）
