华北农学报Issue(1)：137-141,5.DOI:10.7668/hbnxb.2015.01.022
猪伪狂犬病病毒河南分离株gE全基因的克隆与序列分析
Cloning and Sequence Analysis of Complete Genome of gE of Pseudorabies Virus Isolates from Henan
摘要
Abstract
The purpose of this study was to determine whether Pseudorabies virus ( PRV) infection caused by wild strain occurred at the farms where PRV gene deletion vaccine was inoculated under normal immune procedure in some areas of Henan Province and whether variation had occurred in gE gene which was one of the most impor-tant virulence genes of PRV .Samples from swine with suspicious PRV infection were collected from Nanyang , Zhoukou and other regions of Henan and detected by PCR .PRV-positive samples were inoculated into swine testis cells and sub-cultured six times.The complete genomes of gE of 9 PRV strains were cloned ,sequenced and ana-lyzed.The result showed that the length of gE genomes of the 9 strains were 1 862 bp.The homologies between the 9 strains were 99.9%-100% and the homologies with other strains were 97 .5%-99 .6%.Phylogenetic tree showed the 9 PRV strains belonged to a relatively independent sub-branch ,and contained 2 amino acid insertions . The replacement of amino acid occurred at the locus of 448 and the locus of 510 .It indicated that all of the isolated PRV strains were wild isolates ,which were related with the new prevalence .The variation had occurred in gE gene of these isolates .关键词
猪伪狂犬病毒/gE基因/克隆/序列分析Key words
Pseudorabies virus/gE gene/Cloning/Sequence analysis分类
农业科技引用本文复制引用
高晓云,顾阳,潘鑫龙,郭小参,崔保安,陈红英..猪伪狂犬病病毒河南分离株gE全基因的克隆与序列分析[J].华北农学报,2015,(1):137-141,5.基金项目
河南省重大科技专项 （）
