华北农学报Issue(1)：162-164,3.DOI:10.7668/hbnxb.2015.01.027
玉米叶片2种总蛋白质提取方法的比较
Comparison Two Methods of Corn Leaves Total Protein Extraction
摘要
Abstract
To compare the effect of corn leaf protein extraction by different methods with Two-dimensional elec-trophoresis.Two methods of TCA-acetone precipitation and TRIzol Kit were used to extract the leaf genome-wide protein from maize variety Zhengdan 958 ,and the effect of protein extractions were evaluated by two-dimensional e-lectrophoresis .The results showed that the focus voltage of proteins extracted by TCA-acetone method rose slowly and could not be up to the program settings , while the Real-time monitoring map of TRIzol Kit method coincided well with the program settings .After analysis of gel by ImageMaster 2D-Platinum,more than 900 protein spots were separated by Trizol Kit method ,and the low abundance proteins displayed fully .For majority of the protein spots ,the molecular weight (MW) was 20-80 kDa,the pH value was 4-7,and the shape was regular .The protein spots sepa-rated by TCA-acetone precipitation method were 350 only,showing the less protein types separated from a sample , while the high abundance protein over expressed and the detection rate of low abundance proteins reduced greatly .关键词
玉米叶片/蛋白质提取方法/双向电泳Key words
Maize leaf/Extraction methods of protein/Two-dimensional electrophoresis分类
农业科技引用本文复制引用
齐建双,王彦玲,铁双贵,卢彩霞,岳润清,韩小花,燕树锋,孙若楠..玉米叶片2种总蛋白质提取方法的比较[J].华北农学报,2015,(1):162-164,3.基金项目
河南省重大专项 （）
