华北农学报Issue(1)：177-181,5.DOI:10.7668/hbnxb.2015.01.030
N-糖基化对植酸酶phy(QF)酶学性质的影响
The Effects of N-glycosylation on Enzymological Properties of Phytase Phy(QF)
摘要
Abstract
In order to study the effects of N-glycosylation on enzymological properties of phytase phy (QF),the recombinant phytase gene phy(QF) was expressed in pichia pastoris GS115.Phytase phy(QF) was deglycosylated by endoglycosylase Endo H f .Enzymology properties of glycosylated phy (QF)and deglycosylated phy (QF)were an-alyzed.The results showed that the glycosylated phy (QF) had a specific activity of 3 201 U/mg with an MW of ap-proximately 53 kDa and the Km and Vmax of glycosylated phy(QF) were 392 μmol/L and 3 267 U/mg,respectively. The optimal pH value of glycosylated phy(QF) was 4.5 and was consistented with that of deglycosylated phy (QF). However,the optimal temperature of glycosylated phy (QF) was 55 ℃,5 ℃ higher than that of deglycosylated phy (QF) and 2 ℃increased in the melting temperature (Tm).The glycosylated phy(QF) exhibited 13%residual ac-tivity after treatment at 70 ℃for 10 min and deglycosylated phy (QF)was completely deactivated under the condi-tion.As one of posttranslational modifications ,N-glycosylation may play an important role in improving the stability of phytase phy (QF).关键词
植酸酶/N-糖基化/毕赤酵母/热稳定性/圆二色谱Key words
Phytase/N-glycosylation/Pichia pastoris/Thermal stability/Circular dichroism分类
生物科学引用本文复制引用
王茜,宋玛丽,杜军,付月君,胡风云,梁爱华..N-糖基化对植酸酶phy(QF)酶学性质的影响[J].华北农学报,2015,(1):177-181,5.基金项目
国家自然科学基金项目（31372199；31272100）；国家高技术研究发展计划“863”计划项目（2012AA020809）；山西省回国留学人员科研资助项目 （）
