华北农学报Issue(2)：72-77,6.DOI:10.7668/hbnxb.2015.02.014
猪嵴病毒CH441株VP1基因的克隆与序列分析
Molecular Cloning and Sequence Analysis of the VP1 Gene of Porcine kobuvirus
摘要
Abstract
The aim of the study to investigate the main structural protein of the Kobuvirus VP1 gene. According to the sequences of PKV deposited in GenBank,a pair of special primers was designed for amplifying the VP1 gene of swKoV CH441 strain by RT-PCR. The results of sequence analysis showed that the whole VP1 gene of swKoV CH441 strain consisted of 762 bp. Compared with 15 PKV strains which were deposited in GenBank,the homology of nucleotide sequences was 81. 5%~90. 2%,and the homology of deduced amino acids was 86. 6%~96. 9%. Evo-lution analysis indicated that the swKoV CH441 strain was closely related to GS-1 strains. The bioinformatics analy-sis demonstrated that the isoelectric point and molecular weight of non-structural protein VP1 were 4. 40 and 26. 978 2 kDa. The protein had no signal peptide and transmembrane domain. There were 18 phosphorylation sites including 7 Sers,6 Thrs and 5 Tyrs. Protein phosphorylation was concerned with signal transduction,so this protein may be a signaling molecule. The results provided a theoretical foundation for further research on the study of VP1 gene( protein)in the genetic variation.关键词
VP1 基因/克隆/DNA测序/序列分析Key words
VP1 gene/Cloning/DNA sequencing/Sequence analysis分类
生物科学引用本文复制引用
祝俊鹏,杨彬,兰喜,柳纪省,马小军..猪嵴病毒CH441株VP1基因的克隆与序列分析[J].华北农学报,2015,(2):72-77,6.基金项目
国家国际科技合作专项 （）
