华北农学报Issue(2)：145-149,5.DOI:10.7668/hbnxb.2015.02.026
板栗及其近缘种叶绿体SSR遗传多样性分析
Diversity of Cultivated Chestnuts and It′s Close Relative Species Using Chloroplast SSRs
摘要
Abstract
The objective of the study is to elucidate the genetic relationship and genetic diversity of Castanea species. Based on the technology of chloroplast microsatellite markers,of total 24 cpSSR primer pairs screened,only four presented length polymorphism in the Castanea nollissina tested. We surveyed 6 species,altogether 56 materi-als with cpSSR markers in order to estimate genetic variation and genetic structure. In the castanea plants,average number of alleles( Na),effective number of alleles( Ne),expected heterozygosity( He)and Nei′s were 3. 25, 2. 554,0. 606 and 0. 320,respectively. Apparently,the parameters of cpSSR loci were much lower than those re-vealed by nuclear SSRs. Furthermore,the intra-specific genetic diversity was high but varied greatly among different species,the wild chestnut in China showed the highest genetic diversity,which one was rich in heredity and varia-tion. The results have important implications of understanding the population genetics and evolutionary patterns in the genus Castanea and provide baseline data for formulating the conservation and management strategies.关键词
板栗/叶绿体微卫星 DNA/遗传多样性/序列Key words
Castanea nollissina BL./cpSSR DNA/Genetic diversity/Sequence分类
生物科学引用本文复制引用
程丽莉,胡广隆,苏淑钗,黄武刚..板栗及其近缘种叶绿体SSR遗传多样性分析[J].华北农学报,2015,(2):145-149,5.基金项目
“十二五”农村领域国家科技计划专题（2013BAD14B0402-1）；林业公益行业科研专项（201104025）；北京市科委项目 （）
