华北农学报Issue(3)：42-47,6.DOI:10.7668/hbnxb.2015.03.008
高粱苗期耐盐碱性QTL定位
Identification of QTLs Associated with Complex Salt-alkaline Tolerance at the Seedling Stage in Sorghum
摘要
Abstract
Aimed to identify QTLs associated with seedling four traits under complex salt-alkaline stress in Sor-ghum and provide target loci and linked molecular markers for marker assisted selection of Sorghum salt -alkaline tolerant-related traits,an F2 population with 170 lines derived from cross between BJ-299 ×Tx622B were used in this study.Quantitative trait loci (QTLs) for relative livability (RL),relative dry weight (RDW),relative fresh weight (RFW) and relative seedling height (RSH) at seedling stage were analyzed by interval mapping method . The results demonstrated that salt-alkaline tolerance at seedling stage of sorghum was a complex quantitative trait and controlled by multiple genes .Correlation coefficients between each two traits in the F 2∶3 populations under com-plex salt-alkaline stress condition showed significant positive correlation .A total of 3 QTLs ( qSAT-A,qSAT-D and qSAT-J) located on three major chromosome regions ( Sam27281-Sam22486,Sam11433-Sam78379 and Sam62346a-Sam50778) on chromosome A,D and J,respectively.The negative effects were all from the salt-alkaline sensitive parent Tx622 B.qSAT-A and qSAT-D were controlling four salt-alkaline tolerant related traits simultaneously , and explained phenotypic variation ranging from 8 .0% to 18 .8%.QTL qSAT-J for RL was detected with phenotypic variation explained of 7.3%.The two major QTLs (qSAT-A,qSAT-D) and three chromosome regions played crucial role in salt-alkaline tolerance of sorghum , which may have a potential in the practice of marker assisted sorghum breeding for improving salt-alkaline tolerance .关键词
高粱/苗期/耐盐碱/QTL定位/SSR标记Key words
Sorghum/Seedling stage/Salt-alkaline tolerance/QTL mapping/SSR marker分类
农业科技引用本文复制引用
唐朝臣,高建明,韩芸,罗峰,裴忠有,孙守钧..高粱苗期耐盐碱性QTL定位[J].华北农学报,2015,(3):42-47,6.基金项目
天津市科技支撑计划项目（12ZCZDNC00100）；天津市千人计划项目；天津市高校优秀青年教师资助计划项目 （）
