华北农学报Issue(3)：54-57,4.DOI:10.7668/hbnxb.2015.03.010
转 EPSPS 基因抗草甘膦棉花的遗传分析
Genetic Analysis of Transgenic Glyphosate-resistance Cotton with EPSPS Gene
摘要
Abstract
The purpose of this study was to analysis the genetic characters of transgenic glyphosate -resistance cotton with EPSPS gene,using the glyphosate-resistant cotton transformation events G 6-1-G6-26 as the materials, and their non-transgenic genetic background cultivar CCRI-49 as control .Field resistance test in T 1 plants showed that the 20 of 26 transformation events were consistent with the ratio of 3∶1 and the other transformation events were inconsistent with single-gene Mendelian inheritance according to χ2 test.Field resistance test in T 2 plants showed 152 homozygous resistance lines were obtained derived from 25 transformation events ,respectively .Fifty-seven lines derived from 15 transformation events were consistent with the ratio 3∶1 through the analysis of heterozygous resist-ance lines.Furthermore,every transformation event had lines which were inconsistent with the ratio of 3∶1,and 10 transformation events did not have lines which were consistent with the ratio of 3∶1 .These suggested the integration and genetic mode of exogenous gene transformed by the pollen tube pathway method were complex .关键词
棉花/草甘膦/EPSPS基因/遗传分析Key words
Cotton/Glyphosate/EPSPS gene/Genetic analysis分类
农业科技引用本文复制引用
燕树锋,祝水金,刘海芳,卢彩霞,铁双贵..转 EPSPS 基因抗草甘膦棉花的遗传分析[J].华北农学报,2015,(3):54-57,4.基金项目
转基因生物新品种培育重大专项 （）
