华北农学报Issue(3)：123-128,6.DOI:10.7668/hbnxb.2015.03.022
外源NO对铅胁迫下水果黄瓜种子萌发和幼苗生理特性的影响
Influences of Exogenous Nitric Oxide on Seed Germination and Seedling Physiological Characteristics of Fruit Cucumber under Pb2 +Stress
摘要
Abstract
The mechanisms of Cucumis sativus L.seedlings responding to Pb stress were studied in order to pro-vide a theoretical basis for lead-resistant .The effects on the germination of cucumber seeds and the contents of solu-ble protein and MDA ,the activities of POD ,CAT and SOD and chlorophyll relative contents of cucumber seedlings of the different concentration of exogenous NO ( 50 , 100 , 300 , 500 μmol/L ) were studied under 200 mg/L Pb( NO3 ) 2 .The results showed that the germination of fruit cucumber seeds was inhibited significantly ,the contents of soluble protein and MDA increased ,while the activities of POD ,CAT and chlorophyll relative contents decreased obviously under 200 mg/L Pb( NO3 ) 2 .The germination percentage ,germination potential and germination indexes of fruit cucumber seeds were stimulated greatly with adding sodium nitroprusside ( SNP,nitric oxide donor ) ,which de-creased the contents of MDA at the same time ,while raised the activities of POD ,CAT and the contents of relative chlorophyll and soluble protein .It could obviously promote the germination of fruit cucumber seeds under Pb 2+stress with adding exogenous nitric oxide ,enhance the osmotic adjustment capacity of cell ,increase the activity of protective enzyme and significantly alleviate oxidative damage to seeding leaves ,the effect was the best when SNP was at 100 μmol/L especially .关键词
水果黄瓜/一氧化氮/铅胁迫/种子萌发/生理特性Key words
Fruit cucumber/Nitric oxide/Pb2+stress/Seed germination/Physiological characteristics分类
农业科技引用本文复制引用
肖小君,黄作喜,陈文年,王辉..外源NO对铅胁迫下水果黄瓜种子萌发和幼苗生理特性的影响[J].华北农学报,2015,(3):123-128,6.基金项目
四川省教育厅科研项目（10ZA014）；四川省教育厅重大成果转化培育项目 （）
