华北农学报Issue(3)：175-179,5.DOI:10.7668/hbnxb.2015.03.030
黄瓜棒孢叶斑病菌的分子鉴定及 rDNA-ITS 序列分析
Molecular Identification and the Internal Transcribed Spacer Regions Analysis of Pathogen Causing Cucumber Corynespora Leaf Spot
摘要
Abstract
In order to analyze the differentiation of different areas Corynespora cassiicola and identify the patho-gen accurately so as to serve the cucumber disease resistance breeding ,34 corynespora cassiicola purified strains was collected from 2007 to 2014 .Molecular identification results showed that all the isolates were Corynespora cassiicola using published specific primers CCF/CCR, all the 34 strains were amplified the expected 272 bp specific frag-ments.559 bp fragments of ribosomal DNA internal transcribed spacer sequences of corynespora cassiicola were ob-tained through PCR amplification by using universal primers ITS 1 and ITS4.Sequence analysis result showed that there was no difference among the sequences of ribosomal DNA ITS of 32 strains isolated from cucumber ,only 2 nu-cleotides mutation comparing with the sequence of the strains isolated from tomato ,the similarity was 99.64%.The above results showed that the ITS sequences were highly conserved in Corynespora Güssow,the ITS sequence might be used for differential identification of species of Corynespora,the ITS sequence can distinguish different isolates of Corynespora cassiicola fromcucumber and tomato host .关键词
黄瓜/多主棒孢菌/分子鉴定/rDNA-ITSKey words
Cucumber/Corynespora cassiicola(Berk.&Curt.)Wei/Molecular identification/rDNA-ITS分类
农业科技引用本文复制引用
王惠哲,邓强,曹明明,杨瑞环,李淑菊..黄瓜棒孢叶斑病菌的分子鉴定及 rDNA-ITS 序列分析[J].华北农学报,2015,(3):175-179,5.基金项目
天津市自然科学基金项目（13JCZDJC28800）；“十二五”国家科技计划项目 （）
