河北医学Issue(8)：1233-1237,5.DOI:10.3969/j.issn.1006-6233.2014.08.001
大鼠肝细胞癌变过程中 Survivin 与 Apollon的动态表达研究
Dynamic Expression of Survivin and Apollon in Rats During Malignant Transformation
摘要
Abstract
Objective: To determine the dynamic expression and role of survivin and apollon during hepatocellular carcinogenesis ( HCC) in rats.Method:To establish modified DEN-induced primary hepato-carcinoma rat model and detect the protein and mRNA of survivin and apollon in the different pathologic peri-od during hepatocarcinogenesis by using immunohistochemistry and reverse transcriptase -polymerase chain reaction (RT-PCR).Result:The hepatoma model was induced by modified DEN , At the end, four rats died in experimental group, the mortality was 5.88%(4/68).In experimental group,in the sixteen survived rats, fifteen rat induced hepatocarcinoma ,the rate of hepatocarcinoma was 93.75%(15/16).The results of immu-nohistochemistry and RT-PCR shows that the proteins positive expression rate and expression intensity be-came higher and higher ,The difference between groups were statistically significant ( P<0.05) .The expres-sion of survivin was positively correlated with apollon expression in HCC .(r=0.515,p=0.003<0.05).Con-clusion:The expression of survivn and apollon protein is significantly high in human HCC indicating that survivin and apollon play an important role in hepatocarcinogenesis as early diagnostic stage of liver cancer .关键词
大/鼠/肝细胞癌/Survivin/ApollonKey words
Rat/Hepatocellular carcinoma/Survivin/Apollon引用本文复制引用
张隆鑫,王炜..大鼠肝细胞癌变过程中 Survivin 与 Apollon的动态表达研究[J].河北医学,2014,(8):1233-1237,5.基金项目
国家自然科学基金 （）
