河北医学Issue(4)：539-542,4.DOI:10.3969/j.issn.1006-6233.2015.04.004
BMSCs在功能化自组装多肽水凝胶中定向分化神经细胞研究
Directed Differentiation of Bone Marrow Stromal Cells in the Functional Differentiation of Self-assembling Peptide Hydrogel
摘要
Abstract
Objective:To investigate the potential and differentiation of bone marrow stromal cells which in functional self-assembling peptide hydrogel after directed differentiation for neural cells functional charac-teristics.Method:The functionalized self-assembling peptide was produced by mixing the self-assembling peptide RADA16 and peptide RADA16-IKVAV.BMSCs were seeded into RADA 16 self-assembling peptide hydrogel (control group) and functionalized self-assembling peptide hydrogel (experimental group) surface. Pre-inducing agents with bFGF and EGF and directed inducers ( SHH +RA) induced timing , application Nestin, after MAP2, GFAP, ChAT, VAT immunofluorescence staining to detect morphological and function-al differentiation of neural-like cells mark .Result: By inducing BMSCs in functional self-assembling pep-tide hydrogel culture and inducers communist specific combination and timing of the proliferation and differ -entiation of neuronal cells exhibit like change , MAP2-positive cells was significantly increased compared with the control group ( P<0.05) , GFAP-positive cells the percentage was significantly lower than the con-trol group ( P<0.05) , and the feature flag ChAT , VAT expressed only in the experimental group .Conclu-sion:BMSCs in the function of self-assembling peptide hydrogels can be induced into neural cells have cer-tain features.关键词
水凝胶/骨髓基质干细胞/自组装多肽/神经细胞Key words
Hydrogel/Bone marrow stromal cells/Self-assembling peptide/Neural cell引用本文复制引用
李鹏斌,车彪,张振兴,王凯..BMSCs在功能化自组装多肽水凝胶中定向分化神经细胞研究[J].河北医学,2015,(4):539-542,4.基金项目
交通运输部长江航务管理局科技项目基金， （）
