郑州大学学报（医学版）Issue(4)：540-542,3.DOI:10.13705/j.issn.1671-6825.2014.04.027
羧甲基壳聚糖温敏凝胶对 NIH3 T3细胞增殖、骨化分化的影响
Effects of carboxymethyl chitosan thermosensitive hydrogel on the prolif-eration and ossification differentiation of NIH3T3 cells
摘要
Abstract
To explore the effects of carboxymethyl chitosan thermosensitive hydrogel on NIH 3T3 cell prolifera-tion and ossification differentiation.Methods:MTT method and ALP method were used to test the effects of 20, 100, 150, 200, 500 g/L carboxymethyl chitosan thermosensitive hydrogel leaching liquors on NIH 3T3 cell proliferation and ossification differentiation which was cultured in vivo for 24, 48 and 72 h.Results: Both methods showed that OD values of various experimental groups cultured for 24, 48 and 72 h were higher than that of the control group (proliferation:Fgroup =113.961, Ftime =429.535, Finteraction =7.304, P<0.001;ossification differentiation:Fgroup =57.043, Ftime =3 177.618, Finteraction =12.827,P<0.001);150 g/L carboxymethyl chitosan thermosensitive hydrogel leaching liquor had the best cell prolifera -tion and ossification differentiation effects .Conclusion: Carboxymethyl chitosan thermosensitive hydrogel can promote NIH3T3 cell proliferation and ossification differentiation .关键词
羧甲基壳聚糖/温敏性水凝胶/NIH3T3细胞/细胞增殖/细胞分化Key words
carboxymethyl chitosan/themosensitive hydrogel/NIH3T3 cell/proliferation/diferentiation分类
医药卫生引用本文复制引用
陈乃玲,穆玉,李明珠,孙竹梅,徐宝珍..羧甲基壳聚糖温敏凝胶对 NIH3 T3细胞增殖、骨化分化的影响[J].郑州大学学报（医学版）,2014,(4):540-542,3.基金项目
唐山市科技计划项目13130276z （）
