郑州大学学报（医学版）Issue(5)：622-625,4.DOI:10.13705/j.issn.1671-6825.2014.05.007
p16基因高表达对293细胞衰老的影响
Effects of p16 gene overexpression on 293 cells aging
摘要
Abstract
Aim:To investigate the regulation of overexpression of p 16 gene on aging of 293 cells.Methods:The ex-periment had 3 groups:control group,empty plasmid group,and overexpression group.The p16 gene was amplified by RT-PCR to construct the p16 gene overexpression vector ,and the vector was transfected into 293 cells.CCK-8 assay and flow cytometry were performed to detect the effect of p 16 gene overexpression on cell proliferation and cell cycle .β-galactosidase staining was used to detect the cell senescence state ,and the expressions of p 16 and p21 in mRNA and protein level were analyzed by RT-PCR and Western blot .Results:p16 gene overexpression could inhibit the proliferation of 293 cells and the cell cycle was arrested in G0/G1 phase(F=158.057,P<0.001).The expressions of p16 and p21 in the overexpression group increased in the mRNA and protein level (F=73.467,54.988,9.923,44.060,P<0.05),and the percentage of cells staining positively for β-galactosidase increased (F=137.266,P <0.001).Conclusion:p16 gene overexpression could inhibit the proliferation of 293 cells,arrest the cell in G0/G1 phase,improve the expression of p16 and p21,and pro-mote the aging .关键词
p16/高表达/细胞衰老/调控/293细胞Key words
p16/overexpression/cell aging/regulation/293 cell分类
生物科学引用本文复制引用
马珊珊,刘静,姚宁,崔渊博,邢衢,王梦然,郭天宇,杨波,关方霞..p16基因高表达对293细胞衰老的影响[J].郑州大学学报（医学版）,2014,(5):622-625,4.基金项目
国家自然科学基金资助项目81071008；河南省杰出青年基金114100510005；河南省科技攻关项目122102310203；人事部留学回国人员择优资助项目 （）
