郑州大学学报（医学版）Issue(5)：641-644,4.DOI:10.13705/j.issn.1671-6825.2014.05.012
黄芪多糖对人红白血病 K562细胞凋亡的影响
Effect of astragalus polysaccharide on apoptosis of human erythroleukemia K562 cells
摘要
Abstract
Aim:To investigate the effect of astragalus polysaccharide ( APS) on apoptosis of human erythroleukemia K562 cells.Methods:The apoptosis of K562 cells induced by APS was observed by Annexin V-FITC/PI combined with flow cytometry and Caspase-3 activity assay.The expressions of Bcl-2,Bcl-XL,Bax,IAP-1 and Smac in K562 cells(control group)and K562 cells treated with 200 mg/L APS for 48 h(APS group)were detected at the mRNA and protein levels by RT-PCR and Western blot assay,respectively.Results:The number of apoptosis K562 cells in APS group(3 322.000 ± 413.849)was significantly higher than that in control group (101.333 ±18.339).The difference had statistical significance (t=13.466,P<0.001).Compared with the control group ,Caspase-3 activity,Bax and Smac expression in K562 cells of APS group increased significantly(t=11.562,P<0.001),while Bcl-2 and IAP-1 expression decreased significantly(P<0.001),and Bcl-XL expression was not significantly different in K 562 cells of APS group compared with the control group (P>0.05).Conclusion:APS induced apoptosis of K562 cells through down-regulating the expression of Bcl-2 and IAP-1 and up-regulating the expression of Bax and Smac .关键词
白血病/黄芪多糖/凋亡/凋亡相关基因Key words
leukemia/astragalus polydsaccharide/apoptosis/apoptosis-related genes分类
医药卫生引用本文复制引用
李超,钱新华,千新来,付琳琳,吴余..黄芪多糖对人红白血病 K562细胞凋亡的影响[J].郑州大学学报（医学版）,2014,(5):641-644,4.基金项目
河南省基础与前沿技术研究计划基金资助项目122300410121 （）
