郑州大学学报（医学版）Issue(5)：654-657,4.DOI:10.13705/j.issn.1671-6825.2014.05.016
KLK14基因沉默对 OVCAR-3细胞凋亡和迁移能力的影响
Effects of RNA interference targeting kallikrein-related peptidase 14 on apoptosis and migration of OVCAR-3 cells
摘要
Abstract
Aim:To investigate the effect of KLK14 inhibition by RNA interference on the apoptosis and migration in human ovarian carcinoma OVCAR-3 cells.Methods:KLK14 small hairpin RNA(shRNA) was transfected into OVCAR-3 cells with high expression of KLK14(experimental group).The cells transfected with control shRNA (control group) and the cells without treatment ( blank control group ) were the control .RT-PCR and Western blot were used to detect the expressions of KLK14 mRNA and protein .Apoptosis and migration were observed in OVCAR-3 cells by TUNEL and Transwell chamber assay,and the expressions of Caspase-3,Caspase-9,MMP2 and MMP9 were examined by Western blot .Results:Compared with control group and blank control group ,the expressions of KLK14 mRNA and protein in experimental group were de-creased(F=187.861 and 167.374,P<0.05),the apoptosis rate of OVCAR-3 cells was significantly increased ,cell migra-tion was repressed(F=54.313 and 85.283,P<0.05),the expressions of Caspase-3 and Caspase-9 protein were increased (F=139.471 and 91.308,P<0.05),while those of MMP2 and MMP9 were decreased significantly (F=48.219 and 61.941,P<0.05).Conclusion:Inhibition of KLK14 can repress the malignant behavior of OVCAR-3 cells,and KLK14 may serve as a target for ovarian carcinoma therapy .关键词
人组织激肽释放酶14/RNA干扰/OVCAR-3细胞/凋亡/迁移Key words
kallikrein-related peptidase 14/RNA interference/OVCAR-3 cell/apoptosis/migration分类
医药卫生引用本文复制引用
张瑞涛,史惠蓉,任芳,陈志敏,贾艳艳,冯巍..KLK14基因沉默对 OVCAR-3细胞凋亡和迁移能力的影响[J].郑州大学学报（医学版）,2014,(5):654-657,4.基金项目
河南省教育厅自然科学研究项目2011A320045 （）
