郑州大学学报（医学版）Issue(1)：75-78,4.DOI:10.13705/j.issn.1671-6825.2015.01.021
吸入式气管滴注法的建立
Establishment of an inhalable intratracheal instillation
摘要
Abstract
Aim:To establish an inexpensive and high-efficiency method with reference to experiments of dosing or ex-posing through airway .Methods:A total of 24 male BALB/c mice were divided into two groups randomly and , which were nasal drip group and inhalable intratracheal instillation group , respectively;24 male SD rats were divided into two groups randomly , which were non-exposed intratracheal instillation group and inhalable intratracheal instillation group respectively ;12 in each group .Each mouse was instilled with 50μL normal saline and each rat was infused with 100μL.One out of ev- ery group was infused with normal saline configurated with 50% volume fraction of blue ink .Effects of the methods were observed through anatomy.Moreover, operation time that was from the end of anesthesia to the finish of drip and success rate of instillation were recorded .Results:The operation time of inhalable intratracheal instillation group on mice and rats were(25.2 ±4.0) s and (39.9 ±3.3) s which were shorter than that of nasal drip group [(92.6 ±5.4) s] and non-ex-posed intratracheal instillation group [(102.6 ±6.5) s](t=35.042,29.849, P<0.001).It was shown that lung tissue of mice or rats were scattered with blue blots after inhalable intratracheal instillation and non-exposed intratracheal instilla-tion, but it was not obvious after nasal drip .Success rates of nasal drip and inhalable intratracheal instillation were 100%, while that of non-exposed intratracheal instillation was 83.7%.Conclusion:Inhalable intratracheal instillation is inexpen-sive, rapid, non-invasive and high-efficiency, which can be widely applied in kinds of experiments to expose through air-way and establishment of lung disease models .关键词
气管滴注/插管/呼吸道/吸入Key words
intratracheal instillation/intubation tube/airway/inhalation分类
医药卫生引用本文复制引用
晋乐飞,冯斐斐,吴卫东,张巧,姚武,陈小玉,李春阳,徐玉宝,暴磊,郭向娇..吸入式气管滴注法的建立[J].郑州大学学报（医学版）,2015,(1):75-78,4.基金项目
国家自然科学基金资助项目81373030 （）
