郑州大学学报（医学版）Issue(2)：240-243,4.DOI:10.13705/j.issn.1671-6825.2015.02.025
阿司匹林对人卵巢癌组织和 SKOV3细胞 SOX7表达和 Wnt／β-catenin信号通路的影响
Effect of aspirin on expression of SOX7 and Wnt/β-catenin signaling pathway in human ovarian cancer tissue and SKOV 3 cells
摘要
Abstract
Aim:To investigate the effect of aspirin on expression of SOX 7 and Wnt/β-catenin signaling pathway in hu-man ovarian cancer SKOV3 cells.Methods:The expression levels of SOX7,β-catenin and Cyclin D1 in ovarian tumor tissue and normal ovarian tissue were detected by quantitative real-time PCR( qRT-PCR) .The SKOV3 cells were stimulated by dif-ferent concentrations of aspirin in vitro and incubated for 48 h, then the expression of SOX 7,β-catenin and Cyclin D1 mRNA were detected by qRT-PCR.The effect of aspirin on SKOV3 cell proliferation was measured by MTT assay .Results:The ex-pression of SOX7 mRNA was decreased in ovarian cancer tissue (t=3.614,P=0.001),but the expressions of β-catenin and Cyclin D1 proteins were increased(t=13.651, 5.967, P<0.001).The expression level of SOX7 mRNA was increased with the treatment of increasing concentration of aspirin (F=12.151,P<0.001),but those of β-catenin mRNA and Cyclin D1 mRNA were decreased(F=6.214,4.903,P<0.05) in SKOV3 cell.Aspirin inhibited the cell proliferation of SKOV3 cells in a concerntration-dependent manner(F=20.481,P<0.001).Conclusion:Aspirin may have effect on Wnt/β-catenin sig-naling pathway by upregulating the expression of SOX 7 mRNA in human ovarian cancer SKOV3 cells.关键词
SOX7/卵巢癌/SKOV3细胞/阿司匹林/Wnt/β-catenin/Cyclin D1Key words
SOX7/ovarian cancer/SKOV3 cell/aspirin/Wnt/β-catenin/Cyclin D1分类
医药卫生引用本文复制引用
张根豪,刘俊文,苏利沙,刘红春..阿司匹林对人卵巢癌组织和 SKOV3细胞 SOX7表达和 Wnt／β-catenin信号通路的影响[J].郑州大学学报（医学版）,2015,(2):240-243,4.基金项目
河南省教育厅重点科技攻关项目2013020036 （）
