摘要
Abstract
Aim:To study the effects of L-carnitine on the in vitro-fertilized outcomes of mouse oocytes underwent vit-rification.Methods:Oocytes were divided into fresh group (243 survival oocytes), vitrification group(282 thawed oo-cytes), L-carnitine high, medium and low dose (2.0, 1.0, 0.5 g/L) groups(thawed oocytes number was 285,280,279, respectively).The GSH content, ROS level in oocytes and survival rate , fertilization rate, cleavage rate, blastocyst forma-tion rate of oocytes in each group were observed .Results:There were significant differences in the GSH content ,ROS level in the oocytes and the survival rate , fertilization rate, cleavage rate, and blastocyst formation rate among the 5 groups(F=881.290,182.760, and χ2 =113.361,21.560,16.660,9.667,P<0.05).Compared with those of the fresh group , the GSH content was lower ,ROS level was higher in the oocytes of vitrification group , and the survival rate , fertilization rate , and cleavage rate decreased(P<0.05).L-carnitine could increase the GSH content , reduce ROS level in the oocytes un-derwent vitrification, and high dose L-carnitine could improve the survival rate (P<0.05).Conclusion:L-carnitine may improve the outcomes of oocytes which undergo vitrification through antioxidant effects .关键词
玻璃化冷冻/卵母细胞/左卡尼汀Key words
vitrification/oocyte/L-carnitine分类
生物科学
