中医学报Issue(6)：769-771,785,4.
通心络干预的脑微血管内皮细胞条件液对大鼠脑皮层神经元的影响
Effect of Tongxinluo Intervened Brain Microvascular Endothelial Cells Conditioned Medium on Cortical Neuron of Rats
摘要
Abstract
Objective:To explore the effect of conditioned medium of rat brain microvascular endothelial cells on oxidative stress of corti-cal neurons and the protective effect of Tongxinluo( TXL)on it. Methods:Four kinds of rats brain microvascular endothelial cell (BMEC)conditioned cultured medium were prepared:①conditioned medium of normal BMEC(N-CM);②conditioned medium of nor-mal BMEC with drug treatment( NT-CM);③ conditioned medium of ischemic BMEC( I-CM);④ conditioned medium of ischemic BMEC with drug treatment(IT-CM). Each type of conditioned medium were applied to BMEC of normal and sugar oxygen deprivation injury(ischemia)and detected the SOD activity and the MDA content for assessing the effects of these media on oxidative stress of neu-ron. Results:① I-CM could decrease the activity of the normal neurons as well as reduced the SOD activity and increased the MDA con-tent,while this injured effect can be reversed remarkably by IT-CM. ②As compared with those of the normal neuron,I-CM aggravated the injured neuron damage. NT-CM and IT-CM can inhibit the lowered of the injured neuron activity and SOD activity and reduce the MDA content. Conclusion:Rat brain microvascular endothelial cells after injury may cause dysfunction of its paracrine,leading to neuro-nal damage. TXL can protect neurons by regulating the function of this paracrine,which might possibly be related its protective effect on neuron oxidative stress function.关键词
通心络/脑微血管内皮细胞/脑皮层神经元/氧化应激Key words
tongxinluo/brain microvascular endothelial cell/cortex neuron/oxidative stress分类
医药卫生引用本文复制引用
盖聪,李澎涛,孙红梅,李卫红,张振强,李聪,于慧玲,贾静..通心络干预的脑微血管内皮细胞条件液对大鼠脑皮层神经元的影响[J].中医学报,2014,(6):769-771,785,4.基金项目
国家重点基础研究发展计划（973计划）项目（编号2012CB518602）；国家自然科学基金项目 （）
