中医学报Issue(6)：772-775,4.
HPLC-DAD 法同时测定脑健胶囊中4种指标成分含量
HPLC-DAD Simultaneous Determination of Four ComPonents in Naojian CaPsules
刘芳 1王宇红 2蔡川 2向韵 2蔡光先 2王籽又1
作者信息
- 1. 湖南中医药大学，湖南 长沙 410208
- 2. 湖南中医药大学中药粉体关键技术及装备国家地方联合工程实验室，湖南 长沙 410208
- 折叠
摘要
Abstract
Objective:To establish an HPLC-DAD method for acquiring fingerprint of Naojian capsules,also simultaneous determination of ligustrazine,ferulaic acid,senkyunilide A and ligustilide. Methods:The analysis was carried out on Elipse XDB-C18(250 mm × 4. 6 mm,5 μm)column with acetonitrile - 0. 3% acetic acid solution as mobile phase for gradient elution,the column temperature was maintained at 30℃;The flow rate was 1. 0 ml / min;The detection wavelength was set at 295 nm,325 nm,280 nm,328 nm separately. Results:The four compounds in their respective peak areas had good lineatities( R2 ≥0. 999 5)and the average recoveries were 95. 60% ~ 106. 2% ,RSD was within 0. 25% ~ 1. 63% . Conclusion:This method is objective and reliable,which is suitable for total components analysis and evaluation the overall quality of Naojian capsules.关键词
脑健胶囊/HPLC-DAD法/川芎嗪/阿魏酸/洋川芎内酯A/藁本内酯Key words
naojian capsules/HPLC-DAD/ligustrazine/ferulaic acid/senkyunolide A/ligustilide分类
医药卫生引用本文复制引用
刘芳,王宇红,蔡川,向韵,蔡光先,王籽又..HPLC-DAD 法同时测定脑健胶囊中4种指标成分含量[J].中医学报,2014,(6):772-775,4.
