摘要
Abstract
Objective:To observe the influence of Shenyankangfu Tablet on glomerular mesangial cell proliferation and extracellular ma-trix synthesis induced by TGF-beta 1. Methods:In vitro cultured normal rat mesangial cells,divided into normal control group,TGF-beta 1 stimulation group,TGF-beta 1 plus Shenyankangfu Tablet low dose group(100 mg·L - 1 ),middle dose group(200 mg·L - 1 ),high dose group(500 mg·L - 1 ). Used methyl thiazolyl tetrazolium(MTT)respectively to observe the effect of Shenyankangfu Tablet on TGF-beta 1 induced cell viability;Double antibody sandwich ABC-ELISA to detect collagen type I(Col I),fibronectin(FN)content. Results:TGF-beta 1 can significantly increase the activity of glomerular mesangial cells of rats,in the TGF-beta1 stimulation group,the expressions level of Col I and FN were significantly higher than the normal control(P < 0. 05);Those of TGF-beta 1 and nephritis tab-lets in each dose group were lower than TGF-beta 1 stimulation group(P < 0. 05). Conclusion:Shenyankangfu Tablet can inhibit cell vi-ability induced by TGF-beta 1 and glomerular extracellular matrix(ECM)synthesis,which suggest that inhibition of TGF-beta 1 stimu-lates GMC secretion of extracellular matrix is one of the important mechanisms of Shenyankangfu Tablet treatment of chronic glomerular disease.关键词
肾炎康复片/转化生长因子-β1/肾小球系膜细胞/细胞外基质/I 型胶原/纤维连接蛋白Key words
shenyankangfu tablet/transforming growth factor beta 1/glomerular mesangial cells/extracellular matrix/collagen type I/fi-bronectin分类
医药卫生
